The information generated by INVADE is critical to developing vaccines, serological diagnostic assays as well as understanding the potential mechanisms of immune pathogenesis responsible for severe neurological and fatal defects.

Overall Objectives

The INVADE research group is identifying the major T-cell epitopes and cross reactive B-cell epitopes on ZIKV and assessing the role of flavivirus antibodies in protective and pathogenic immunity following ZIKV through antibody dependent enhancement (ADE).

Specific Objectives

  • Define the epitope breadth/repertoire and the targets of CD4 and CD8 responses,
  • Derive sets of HLA class I and class II predicted epitopes covering the entire ZIKV proteome,
  • Define phenotypes and T cell subsets associated with CD4 and CD8 specific T cells,
  • Manufacture a representative library of about 2,000 peptides derived from Zika,
  • Identify the major type-specific and cross reactive B-cell epitopes on ZIKV,
  • Assess the role of flavivirus antibodies in protective and pathogenic immunity (e.g. related to prior dengue infections, dengue and yellow fever vaccines),
  • Assess putative in vivo mutations within ADE associated proteins using protein modelling and monoclonal antibody (mAb) mapping.

Participating Organisations

Group leader: Prof. Andrew Falconar, Fundación Universidad del Norte

  • London School of Hygiene & Tropical Medicine
  • La Jolla Institute for Allergy and Immunology
  • The University of North Carolina at Chapel Hill