Gary Kobinger and Trina Racine (eds.), Zika Virus: Methods and Protocols, Methods in Molecular Biology, Chapter 14; Springer Science+Business Media - May 5, 2020
Cheikh Tidiane Diagne, Martin Faye, Benjamin Lopez-Jimena, Ahmed Abd El Wahed, Cheikh Loucoubar, Cheikh Fall, Giulia Mencatelli, Oumar Faye, Ousmane Faye, Manfred Weidmann, and Amadou Alpha Sall
Early diagnostics of Zika virus (ZIKV) is crucial for prognosis, management of patients, and implementation of public health measures. During the past decade, significant progress has been made in ZIKV diagnostic methods as alternatives to real-time quantitative PCR. Among them are loop-mediated isothermal amplification (LAMP) and recombinase polymerase amplification (RPA), both of which have demonstrated high sensitivity and specificity.
The purpose of the present chapter is to compare the methods established for the detection of Zika virus including qRT-PCR, 2 RT-LAMP assay, and RT-RPA. First, the authors describe the techniques, sensitivity, specificity, and clinical validation approaches for the three assays; and second, they compare their performance on a set of ZIKV isolates from the African and Asian/American lineages.