Emerging Infectious Diseases, November 2017
Carlo Fischer, Maria C. Torres, Pranav Patel, Andres Moreira-Soto, Ernest A. Gould, Rémi N. Charrel, Xavier de Lamballerie, Rita Maria Ribeiro Nogueira, Patricia C. Sequeira, Cintia D.S. Rodrigues, Beate M. Kümmerer, Christian Drosten, Olfert Landt, Ana Maria Bispo de Filippis, Jan Felix Drexler
Abstract
The current yellow fever outbreak in Brazil prompted widespread yellow fever virus (YFV) vaccination campaigns, imposing a responsibility to distinguish between vaccine- and wild-type YFV-associated disease. We developed novel multiplex real-time reverse transcription PCRs that differentiate between vaccine and American wild-type YFV. We validated these highly specific and sensitive assays in an outbreak setting.